Familial Hypercholesterolemia (FH) is a disease that causes an increase in blood levels of the "bad" form of cholesterol, known as low density lipoprotein (LDL), and is a serious risk factor for coronary heart disease and stroke. In this experiment, a simulated genetic test for FH is demonstrated in which patients are tested for a DNA polymorphism linked to the FH gene. This complete kit includes detailed instructions along with Ready-to-Load™DNA samples, UltraSpec-Agarose™ powder, practice gel loading solution, electrophoresis buffer, InstaStain® Blue and FlashBlue™ stain, calibrated pipette, 100 ml graduated cylinder, and microtipped transfer pipettes.
Requires the use of an electrophoresis apparatus, power supply, automatic micropipette and tips, balance, microwave or hot plate, and visualization system. Materials provided for six gels. Time Requirements: 60 minutes
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