Immunoblot, otherwise known as dot blot, is a technique used to determine the presence of an antigen. In this experiment, antigens are absorbed to a membrane that is then treated sequentially with an antigen-specific antibody solution and a secondary antibody conjugated to an enzyme. The enzyme-substrate reaction generates a color product that precipitates on the membrane, indicating a positive reaction. This process highlights how clinical diagnostic kits employ the principles of the immuno-blot. No human serum is used in this experiment. Includes materials for six setups. Note: Contents are shipped ambient, some components require storage at 4°C.
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