Students will discover the relationship between genotype and phenotype as they perform a Polymerase Chain Reaction (PCR) using DNA extracted from Edvotek® QuickPlants™. Unlike the wild type QuickPlants™, the glabra mutant lacks the single-haired trichomes on its leaves. Through practical applications of PCR, students will see this distinction at the DNA level as they compare the difference between the glabra mutant and the wild type plants.
Kit includes everything that is needed to grow and cultivate QuickPlants™, including seeds, potting soil pellets, and pots. Also included are PCR beads, DNA extraction buffer, microtubes, primers, plant homogenization pestles and tubes, agarose, electrophoresis buffer, DNA ladder, and FlashBlue™ stain. Required but not included are micropipettes to measure between 5 and 50 µl, tips, waterbath, thermal cycler, electrophoresis apparatus and power supply. Materials are supplied for ten groups, and detailed instructions are provided.
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