Genetic and environmental mutations in the p53 gene, a key suppressor gene essential for cell function, can be correlated to predisposition for certain types of cancers. In this experiment, students will use Ready-to-load™ Predigested DNA (non-human) to study family pedigree determination of several generations relating to cancer formation due to p53 gene mutation. Kit includes activity instructions, Ready-to-load™ Predigested DNA samples, UltraSpec-Agarose™powder, practice gel loading solution, electrophoresis buffer, InstaStain® Ethidium Bromide, pipette, and five autoradiograms.
Requires an electrophoresis apparatus and power supply, automatic micropipette with tips, balance, microwave oven or hot plate, water bath (65ºC), UV transilluminator, pipette pump or bulb, 250 ml Flasks, and distilled or deionized water. Materials supplied for six groups. Time requirements: 60 minutes.
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