In this experiment, students will learn the basic concepts of PCR by observing the increased accumulation of product with increasing number of cycles. The experiment is divided into three modules - PCR Reaction, Separation of PCR Reactions by Electrophoresis, and Size Determination of PCR Amplified DNA Fragment. In this experiment the template DNA to be amplified is a linear molecule from the E. coli bacteriophage lambda. It contains approximately 49,000 base pairs and has 5 recognition sites for Eco RI, and 7 for Hind III. This experiment contains reagents for 10 groups to perform the PCR reactions.
Kit contents:
- Features PCR Dryzyme® reaction pellets™
- Instastain® Ethidium Bromide DNA stain
- UltraSpec-Agarose™
- Electrophoresis Buffer (50x)
- 10x Gel Loading Solution
- InstaStain® Ethidium Bromide
- Microcentrifuge Tubes (0.5 ml)
- PCR tubes (0.2 ml)
- Wax beads (for waterbath option or thermal cyclers without heated lid)
- Coupon for a freezer pack with the following reagents (ship and store at -20°C):
- Primer Mix (two primers)
- 200 base pair ladder
- Lambda DNA Template for Amplification
Requires, but does not include include a horizontal gel electrophoresis apparatus, DC power supply, thermal cycler, balance, microcentrifuge, waterbath, UV transilluminator or white light, microwave, automatic pipets, distilled or deionized water, and isopropynol.
Email this Product to a Colleague
