This pAmylase was created by inserting the amylase gene into pUC 18 plasmid vector. The pUC vector also contains the Ampicillin resistance gene (AmpR), yielding two selection criteria for bacteria transformed with this plasmid „ amylase production and ampicillin resistance.
The pamylase at 0.2 ug/ul concentration is used as a known sample, or standard for DNA electrophoresis in labs 8b, 8f, and 8g from Biotechnology: Science for the New Millennium curriculum. The molecular weight is 6700 bp with a single site for Hind III digestion and three sites for Bam HI.
The pAmylase at 0.005 ug/ul concentration is used in lab 8c from Biotechnology: Science for the New Millennium curriculum to transform JM109 bacteria.
The transformed bacteria are later used to demonstrate scale up prodcution of transformed bacteria, and miniprep techniques using lysozyme digestion and alkaline cell lysis.
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