Performing procedures used in routine screening tests for waterborne bacteria in drinking water, students use PCR in a multiplexed format. Using a single PCR reaction, students test for the presence of three separate, classroom-safe microorganisms. For ten experiments with 20 students.
Kit contents:Primer mixtures
200 base pair ladder
Control DNAs
Non-pathogenic E. coli, B. subtilis, and S. marcescens
Chelating agent
Proteinase k
PCR beads (Taq polymerase, dNTPs, buffer)
UltraPure H2O
TE buffer
10x gel loading solution
InstaStain™ Ethidium Bromide
InstaStain™ Methylene Blue
Microtest tubes with attached caps
Instructions with background information
Requires, but does not include, thermal cycler, waterbaths, or heating baths for PCR, horizontal gel electrophoresis apparatus, DC power supply, micropipettors with tips, balance, 500mL flask, microcentrifuge, UV transilluminator or white light, and microwave.
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