Protein identification is based on both antibody reactions and antigens in this Western Blot activity. The proteins are separated using a denaturing SDS polyacrylamide gel and transferred or blotted to a nylon membrane. The membrane is then exposed sequentially to solutions containing the primary antibody, followed by a secondary antibody coupled to an enzyme. The membrane is soaked in a substrate solution to develop the color reaction, which results in identifying the antigen as a band, and the molecular weights of the visible bands are measured suing prestained protein markers. Materials included for six blots. Note: Shipped ambient. Store lypholized proteins at room temperature.Kit contents:Negative lypholized controlBSA, high and low concentration (lypholized)Anti-BSA protein antibodySecondary antibody conjugateHydrogen peroxidePeroxide co-substrate10x blocking bufferPBS bufferPrestained protein standard marker (lypholized)Tris-glycine-SDS electrophoresis bufferPowdered milkInstructions with background informationRequires, but does not include three polyacrylamide gels, MV-10 vertical gel electrophoresis apparatus, DC power supply, automatic micropipet, fine micropipet tips, glacial acetic acid, methanol, and distilled or deionized water.
Email this Product to a Colleague
